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Dehaasia is a member Lauraceae. It is locally known as ‘gajus hutan’ or ‘pekan’. A triterpenoid, lupeol was isolated from the bark of Dehaasia cuneate. The structure of the isolated compound was determined using spectroscopic methods, such as UV–vis, FT-IR, 1D and 2D NMR, and mass spectrometer. The isolated compound was tested against Gram-negative and positive bacteria using agar disc diffusion technique. The results showed that lupeol had a moderate inhibition zone value of 10.0±0.00 mm against Gram-negative Serratia marcescens ATCC 14756 whereas low inhibition which is 7.0±0.00 mm against Escherichia coli ATCC 25922, Vibrio fluvialis ATCC 33809, Bacillus subtilis ATCC 6633, and Methicillin-resistant Staphylococcus aureus (MRSA) ATCC 43300.